Performing a Bradford Assay

“You conduct a Bradford Assay and measure the optical density of your unknown protein sample to be 0.5 at 595 nanometers. If your standard curve indicates that an optical density of 0.5 corresponds to a protein concentration of 1.5 micrograms per microliter, what conclusion can you draw about the protein levels in your sample? Explain your reasoning. After preparing a dilution series of BSA for a Bradford Assay, you measure the optical densities and create a standard curve. If the optical density of a sample is measured at 0.8 and the standard curve shows that this corresponds to 2.5 micrograms per microliter, what does this indicate about the protein concentration in your sample? Provide a detailed explanation.”