Performing a Bradford Assay
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Objective
I can accurately conduct a Bradford Assay and analyze the data to draw conclusions about protein levels.
Part 1 of 3
Warm-up video
Unknown Channel · 5:00
We don't generate video — this one is by Unknown Channel on YouTube. The practice questions and exit ticket below were drafted by AI for this objective, and every question is editable in the teacher guide.
Part 2 of 3
Practice
12 questions
What is the principle behind the Bradford Assay and how does it allow for protein quantification?
Describe the role of Coomassie Brilliant Blue dye in the Bradford Assay. How does it interact with proteins?
Part 3 of 3
Exit ticket
Quick comprehension check
“You conduct a Bradford Assay and measure the optical density of your unknown protein sample to be 0.5 at 595 nanometers. If your standard curve indicates that an optical density of 0.5 corresponds to a protein concentration of 1.5 micrograms per microliter, what conclusion can you draw about the protein levels in your sample? Explain your reasoning. After preparing a dilution series of BSA for a Bradford Assay, you measure the optical densities and create a standard curve. If the optical density of a sample is measured at 0.8 and the standard curve shows that this corresponds to 2.5 micrograms per microliter, what does this indicate about the protein concentration in your sample? Provide a detailed explanation.”
Sample answer included in the free materials
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