Interpreting Gel Electrophoresis

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Objective

I can interpret gel electrophoresis results to draw conclusions about DNA samples.

Part 1 of 4

Warm-up video

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We don't generate video โ€” this one is by Amoeba Sisters on YouTube. The practice questions and exit ticket below were drafted by AI for this objective, and every question is editable in the teacher guide.

Part 2 of 4

Key concepts

3 concepts

  1. 1

    Gel electrophoresis separates molecules, especially DNA, based on their size and charge.

  2. 2

    Restriction enzymes are used to cut DNA into fragments at specific sequences, and these fragments are then loaded into the wells of the gel.

  3. 3

    DNA bands can be compared to a DNA ladder, which contains fragments of known sizes, to estimate the size of unknown DNA fragments and determine relatedness between samples.

Part 3 of 4

Practice

11 questions

1

What property of DNA allows it to be separated using gel electrophoresis?

2

Explain the role of restriction enzymes in preparing DNA samples for gel electrophoresis.

Part 4 of 4

Exit ticket

Quick comprehension check

โ€œA gel electrophoresis is run on four DNA samples. Sample 1, Sample 2, and Sample 3 are from different individuals. Sample 4 is a DNA ladder with known fragment sizes. After running the gel, the banding patterns for Samples 1 and 3 appear most similar. What can you conclude about the relationship between the individuals who provided Sample 1 and Sample 3?โ€

Sample answer included in the free materials

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