Making Agarose Gels

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Objective

I can prepare an agarose gel for electrophoresis.

Part 1 of 4

Warm-up video

Professor Dave Explains · 5:17Vetted channel

We don't generate video — this one is by Professor Dave Explains on YouTube. The practice questions and exit ticket below were drafted by AI for this objective, and every question is editable in the teacher guide.

Part 2 of 4

Key concepts

3 concepts

  1. 1

    Gel electrophoresis is a method used to separate large molecules like segments of DNA.

  2. 2

    In gel electrophoresis, smaller DNA molecules travel greater distances through the gel, while larger ones travel shorter distances.

  3. 3

    After separation, a DNA-binding dye is added, which glows a fluorescent pink in UV light, allowing visualization of DNA bands.

Part 3 of 4

Practice

12 questions

1

What is the primary purpose of gel electrophoresis?

2

In gel electrophoresis, DNA molecules move towards which electrode and why?

Part 4 of 4

Exit ticket

Quick comprehension check

Describe the composition of the gel used in gel electrophoresis and its purpose in the separation process.

Sample answer included in the free materials

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Teacher guide, student handout, and slides — free, in Google Docs format

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